Enzyme-mediated protein refolding.
نویسندگان
چکیده
We employed a urease-catalyzed reaction to gradually remove a high concentration of a chaotropic agent (urea) from a denatured protein solution and demonstrated that efficient protein refolding can be achieved by the urease-catalyzed reaction, without large-volume dilution.
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ورودعنوان ژورنال:
- Chemical communications
دوره 46 شماره
صفحات -
تاریخ انتشار 2009